Immunofluorescence database
Database 3 - BrdU-NeuN cells in adult rat anterior neocortex (red BrdU, green NeuN)
Adult male rats (Rats 1,2) - perfused 4 weeks after BrdU injection
To view database 3, first download the following free program: Zeiss LSM Image Browser (this is a Windows based program for PC users). Next, left-click Database 3 , which will call up a file download window. Save the compressed database to a local folder on your computer and decompress using WinZip Self-Extractor, StuffIt Expander or other standard software. The folder will then contain a database file (.mdb) and its associated individual .lsm image files. Access the database using Zeiss LSM Image Browser. The browser will allow you to view all z-sections throughout the double-labeled cell(s) (gallery), examine the cells in separate channels (split xy), and measure the size of objects in the image (overlay). Detailed information about the confocal microscope settings used for each scan is included (click the info button).
Merged views |
Single channels - NeuN (green), BrdU (red) |
Notes |
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This BrdU-NeuN cell (arrow) exhibits no NeuN stained cytoplasm. An additional BrdU-NeuN cell is located to the lower left of this frame at a different plane of focus- this can be viewed in Zeiss LSM file. To view the raw data from this confocal image, download Database 3 and open the file entitled:
"Rat1BrdUNeuNantcx1" |
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This BrdU-NeuN cell (arrow) is relatively small and exhibits no NeuN stained cytoplasm. To view the raw data from this confocal image, download Database 3 and open the file entitled:
"Rat1BrdUNeuNantcx2"
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This BrdU-NeuN cell (arrow) is small and has no NeuN stained cytoplasm. To view the raw data from this confocal image, download Database 3 and open the file entitled:
"Rat1BrdUNeuNantcx3" |
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This BrdU-NeuN cell (arrow) is small and exhibits very little NeuN stained cytoplasm. Two additional BrdU-NeuN cells are located to the left of this cell at different planes of focus- these can be viewed in the Zeiss LSM file. To view the raw data from this confocal image, download Database 3 and open the file entitled:
"Rat1BrdUNeuNantcx4" |
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This BrdU-NeuN cell (arrow)is small and exhibits no NeuN stained cytoplasm. An additional BrdU-NeuN labeled cell is located to the left of this cell and can be viewed in the Zeiss LSM file. To view the raw data from this confocal image, download Database 3 and open the file entitled:
"Rat1BrdUNeuNantcx5" |
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This BrdU-NeuN cell (arrow) exhibits no NeuN stained cytoplasm. To view the raw data from this confocal image, download Database 3 and open the file entitled:
"Rat1BrdUNeuNantcx6" |
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This BrdU-NeuN cell (arrow) exhibits no NeuN stained cytoplasm. To view the raw data from this confocal image, download Database 3 and open the file entitled:
"Rat2BrdUNeuNantcx1" |
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This BrdU-NeuN cell (arrow) exhibits no NeuN stained cytoplasm. To view the raw data from this confocal image, download Database 3 and open the file entitled:
"Rat2BrdUNeuNantcx2" |
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This BrdU-NeuN cell (arrow) exhibits no NeuN stained cytoplasm. To view the raw data from this confocal image, download Database 3 and open the file entitled:
"Rat2BrdUNeuNantcx3" |
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